Bovine Testosterone(T) ELISA Kit

Mfr.No.

CSB-E13194B

Sensitivity

0.05 ng/mL

Detection Range

0.1 ng/mL-20 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Testosterone(T)

Recovery

The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	81-99
1:2000	Average %	95
	Range %	83-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	90
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Testosterone

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tadalafil modulates aromatase activity and androgen receptor expression in a human osteoblastic cell in vitro model Aversa A. et al,J Endocrinol Invest.,2015 Jul 2. Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls Nitika S. Jalmeria, et al,Biological rhythm research,December 11 Development of Marbling in Beef Cattle Can Be Modulated through the Control of Adipogenic Activity Theresia Galuh Wandita, et al,/,November 30

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