Bovine Heat Shock Protein Beta-1

Mfr.No.

E2214Bo

Alternative Names

Heat shock 27 kDa protein;Heat shock protein beta-1;HSP27;HSPB 1;HSPB1

Reactivity

Bovine

Detection Range

0.05-30ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22393

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/mL

Background

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

Gene ID	516099
UniProt ID	Q3T149
Antigen	HSPB1

Research Area

Signal Transduction; Cancer; Cardiovascular

Protocol

These standard curves of E2214Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/mL	2.286	2.2	2.147
	2.114
8ng/mL	1.562	1.632	1.58
	1.702
4ng/mL	1.165	1.094	1.041
	1.022
2ng/mL	0.737	0.739	0.686
	0.74
1ng/mL	0.469	0.477	0.424
	0.485
0ng/mL	0.062	0.053	0
	0.043

Precision

We measured random samples of E2214Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.7	0.12	7.4
Intra-Assay	2	18	5.6	0.27	4.8
Intra-Assay	3	18	3	0.23	7.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100508	E2214Bo	96T	$458		Add to Cart
BHE12100508	E2214Bo	48T	$320		Add to Cart

Bovine Heat Shock Protein Beta-1 - HSPB1 ELISA Kit

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