Bovine Fetuin B - FETU-B ELISA Kit

Mfr.No.

E2017Bo

Description

This sandwich kit is for the accurate quantitative detection of Fetuin B (also known as FETU-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FETUB;Fetuin-B;Gugu

Reactivity

Bovine

Detection Range

5-2000ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42663

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.48ng/ml

Background

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity).

Gene ID	504615
UniProt ID	Q58D62
Antigen	FETUB

Research Area

Cardiovascular

Protocol

These standard curves of E2017Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/ml	2.16	2.166	2.111
	2.173
600ng/ml	1.674	1.615	1.56
	1.556
300ng/ml	1.195	1.12	1.065
	1.044
150ng/ml	0.647	0.647	0.592
	0.648
75ng/ml	0.456	0.426	0.372
	0.397
0ng/ml	0.056	0.055	0
	0.054

Precision

We measured random samples of E2017Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	162.9	10.93	6.7
Intra-Assay	2	18	516.3	22.82	4.4
Intra-Assay	3	18	346.7	25.51	7.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100398	E2017Bo	96T	$458		Add to Cart
BHE12100398	E2017Bo	48T	$320		Add to Cart

Bovine Fetuin B - FETU-B ELISA Kit

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