Matrices listed below were spiked with certain level of AA and the recovery rates were calculated by comparing the measured value to the expected amount of AA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
93
EDTA plasma(n=5)
95-103
99
heparin plasma(n=5)
86-97
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.