Cortisol EIA Kit

Mfr.No.	SKT-201
Alternative Names	(11β)-11,17,21-trihydroxypregn-4-ene-3,20-dione EIA Kit,, (11β)-11 ; 17 ; 21-trihydroxypregn-4-ene-3 ; 20-dione EIA Kit
Categories	EIA Kits
Description	Quantitative colorimetric detection of cortisol
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	300
Reactivity	Species independent
Scientific Background	Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance (1). In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization (2). Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the moing and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin (1,3). Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer (4), depression (5), and schizophrenia (6). It is already known that abnormal levels of cortisol are involved in Cushing's Syndrome and Addision's disease (7).
Size	1 Plate ; 125 µl ; 3 ml ; 3 ml ; 28 ml ; 1 ml ; 30 ml ; 11 ml ; 5 ml ; 1 Each
Adr Code	Non-hazardous
Assay Overview	The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Assay Range	100 - 3200 pg/ml
Assay Type	Competitive EIA (Enzyme Immunoassay)
Assay Type Abbreviation	Competitive
Component Number	SKC-201A ; SKC-201B ; SKC-201C ; SKC-201D ; SKC-201E ; SKC-201F ; SKC-201G ; SKC-201H ; SKC-201I ; SKC-201J
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	100 - 3200 pg/ml
Incubation Time	90 Minutes
Item	Clear Microtitre 96 well Plate ; Cortisol Standard ; StressXpress® Cortisol Antibody ; StressXpress® Cortisol Conjugate ; Assay Buffer ; Dissociation Reagent ; Wash Buffer Concentrate ; TMB Substrate ; Stop Solution ; Plate Sealer
Method Type	Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media
Number Of Samples	40 samples in duplicate
Platform	Microplate
Precision	Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV
Sensitivity	17.3 pg/ml
Species Reactivity Abbreviations	ALL
Species Reactivity Medium	Species Independent
Trademark	StressXpress®

Target	Cortisol
Research Area	Cell Signaling Cancer Oxidative Stress Neuroscience
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: EIA kit used to quantitatively measure cortisol present in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-201A	Clear Microtitre 96 well Plate	1 Plate
SKC-201B	Cortisol Standard	125 µl
SKC-201C	StressXpress® Cortisol Antibody	3 ml
SKC-201D	StressXpress® Cortisol Conjugate	3 ml
SKC-201E	Assay Buffer	28 ml
SKC-201F	Dissociation Reagent	1 ml
SKC-201G	Wash Buffer Concentrate	30 ml
SKC-201H	TMB Substrate	11 ml
SKC-201I	Stop Solution	5 ml
SKC-201J	Plate Sealer	1 Each

Incubation Time: 90 Minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 300
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

EIA kit used to quantitatively measure cortisol present in samples.

Reference	1. E. Friess, et al., Eur J Clin Invest, 2000, 30, Suppl 3:46-50. 2. Freeman, Scott, 2002. Biological Science. Prentice Hall; 2nd Pkg edition (December 30, 2004). 3. C. Longscope., J. Endocrinology, 1996, , Suppl S125-S127. 4. J. Herbert, Lancet, 1995 345, 1193-1194. 5. A. Michael, et al., Biol. Psychiatry, 2000, 48, 989-95. 6. C.R. Dequet and D.J. Wallace, Current Opin. Ivest. Drugs, 2001, 8, 1045-53. 7. W.M. Jeffries, Med. Hypotheses, 1998, 51, 114-4.

Other Resources 1	Kit Booklet
Other Resources 2	MSDS
Other Resources 3	Steroid Solid Extraction Protocol
Other Resources 4	Saliva Sample Handling Instructions

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900030-1	SKT-201-96	96 well	$293		Add to Cart

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