Shrimp Alkaline Phosphatase (SAP) (1 U/uL)

Product description

Alkaline Phosphatase, can remove the 5' phosphate groups from DNA and RNA. It is commonly used to prevent the self-ligation of vectors. In molecular cloning experiments, DNA ligase requires the presence of phosphate groups to catalyze DNA ligation. After digestion with restriction enzymes, vectors retain a phosphate group at the cleavage site. However, after dephosphorylation by Alkaline Phosphatase, the 5' end of the vector lacks a phosphate group and thus cannot connect with its own 3' end. As a result, the self-ligation of the vector, which would otherwise preferentially occur in the ligation reaction, is prevented. This increases the insertion rate of the target fragment. Additionally, Alkaline Phosphatase can be used to prepare DNA templates for 5' end labeling.

This product is a shrimp-derived Alkaline Phosphatase that can degrade almost all phosphate monoesters. However, it cannot hydrolyze phosphate diesters or triesters. The enzyme acts on the terminal dephosphorylation, regardless of whether the termini are sticky or blunt. SAP can also be used to degrade dNTPs in PCR reactions, which is useful for subsequent sequencing and SNP analysis.

Features

• • Order
  • Documentation
  • Prerequisite
  • Q & a

  Combination SKU	Supplier No.	Size	Your price	Quantity
  BHS20800179-1	10322ES72	250 U	$155		Add to Cart