Hieff Superfast DNA Methylation Bisulfite Kit

Mfr.No.	12225ES10, 12225ES50
Description	The Hieff Superfast DNA Methylation Bisulfite Kit (Column-based) rapidly converts unmethylated cytosines in DNA samples to uracil, while leaving methylated cytosines unchanged. During high-temperature bisulfite treatment, double-stranded DNA denatures into single strands. In the presence of HSO3-, cytosine residues undergo deamination and are converted into uracil, with methylated cytosines remaining unaltered. In subsequent PCR amplification, uracil is replaced by thymine (T). The conversion process takes only 5 minutes, accommodating DNA input ranging from 100 pg to 2 ug, and achieves a conversion efficiency of >=99% for unmethylated cytosines. The converted DNA is suitable for downstream applications such as PCR amplification and NGS sequencing.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHN20800024-1	12225ES10	10T	$150		Add to Cart
BHN20800024-2	12225ES50	50T	$395		Add to Cart