FuGENE® 4K DNA Transfection Reagent

Mfr.No.	4K-1000, 4K-5000, 4K-10000, 4K-25000, 4K-50000, 4K-250000
Description	FuGENE® 4K is our newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into both challenging and routine mammalian cell lines. FuGENE® 4K was developed to improve upon our widely published and trusted FuGENE® HD reagent. Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE® 4K provides efficient and safe entry of DNA into a wide range of cells. This mechanism allows users to obtain higher performance than other competitor reagents including Lipofectamine® 3000.
Appearance	FuGENE® 4K reagent outperforms Lipofectamine® 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.

Storage Temperature	Product to be stored for long-term storage at 4 degrees Celsius for up to 2 years from date of delivery. Product can be shipped at ambient temperature.

Protocol	Microsoft Word - Datasheet_4K (fugene.com) Microsoft Word - FuGENE 4K SDS (GHS FORMAT)

FuGENE® 4K reagent outperforms Lipofectamine® 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.

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Combination SKU	Supplier No.	Unit Size	Your price
BHN17700001-1	4K-1000	1ml	$538	Add to Cart
BHN17700001-2	4K-5000	5ml	$2465	Add to Cart
BHN17700001-3	4K-10000	10ml	$4437	Add to Cart
BHN17700001-4	4K-25000	25ml	$10501	Add to Cart
BHN17700001-5	4K-50000	50ml	$20382	Add to Cart
BHN17700001-6	4K-250000	250ml	$93912	Add to Cart

FuGENE® 4K DNA Transfection Reagent

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