EXOSOME ISOLATION (PLASMA, URINE)

Mfr.No.	EXPL-0100
Description	SubXTM-Exo kit is designed for isolation of exosomes from liquid biopsies (plasma, serum, etc.). Our proprietary bi-functional substance (SubXTM) interacts with exosomes via phospholipids. Each molecule of SubXTM can anchor two exosomes and thus produce dimers and then oligomers of the extracellular vesicles. SubXTM can oligomerize exosomes in both highly proteinaceous bioliquids (serum, plasma) and in high salt biofluids (urine, tears, etc.). Excess of SubXTM results in oligomerization of up to 10-15 exosomes and formation of micron-size particles that are easily precipitated in a brief 14K x g centrifugation step. A specially designed buffer allows for reconstitution of the pelleted exosomes back to free monomer state suitable for downstream applications. • SubX^TM-Exo Plasma isolation kit Description Designed for isolation of exosomes from plasma. Includes reagents sufficient for up to 100 isolations from 1 ml plasma samples. • SubX^TM-Exo Urine Isolation kit Description Designed for isolation of exosomes from urine samples (up to 5 ml volume). • SubX^TM-Exo-DNA isolation kit Description SubX^TM-Exo-DNA kit is designed for simultaneous isolation of exosomes and circulating cell-free DNA (cfDNA) directly from liquid biopsies (plasma or serum) without Proteinase K. Excess of SubX^TM molecules in the solution results in oligomerization of up to 10-20 exosomes and formation of micron-size particles that are easily precipitated in a brief 14K x g centrifugation step. A specially designed buffer allows for reconstitution of the pelleted exosomes back to free monomer format suitable for downstream applications.

SubXTM binding with exosomes in urine. SubXTM particles size is around 3 nm (blue line). Urine (5,000 rpm supernatant) exosomes have a bimodal distribution of 40 nm peak and 220 nm peak (red line). [Urine (100ul) + SubXTM (20ul)] suspension (green) also have a (shifted) bimodal distribution of minor 210 nm peak and main 1000 nm peak of particle aggregates.

Snapshot images of exosomes taken from NanoSight NS300 videos. A - exosomes in urine; B –SubXTMoligomerizes exosomes to form large complexes with size range >400 - 1200 nm; C – exosomes reconstituted in ERB solution, size within the range 50-120nm

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Combination SKU	Supplier No.	Size	Sample	Your price
BHD17200002-1	EXUR-0100	100 preps	Urine	-	Add to Cart
BHD17200002-2	EXOD-0050	50 preps	DNA	-	Add to Cart
BHD17200002-3	EXPL-0100	100 preps	Plasma	-	Add to Cart

EXOSOME ISOLATION (PLASMA, URINE)

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