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Home / Primary Antibodies / Monoclonal Antibodies

DMPO Antibody, Clone N1664A (SMC-189)

BHA11901415
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+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Antigen DMPO
Target DMPO
Clone No N1664A
Research Area Cancer, Oxidative Stress, Cell Signaling, Metabolism, Mitochondrial Metabolism, Mitochondrial Markers, Redox Metabolism
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Shipping Temperature Blue Ice or 4°C
Specificity Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications AM, ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA ; AM
Dilution WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25ug); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141.

2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522.

3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481.

4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75.

5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436.

6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256.

7. Free Radic Biol Med. (2009) April 1; 46(7): 853–865. doi:10.1016/j.freeradbiomed.2008.12.020.

8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11901415-1 SMC-189D Unconjugated 100 ug $348
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BHA11901415-2 SMC-189D-A390 ATTO 390 100 ug $403
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BHA11901415-3 SMC-189D-A488 ATTO 488 100 ug $402
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BHA11901415-4 SMC-189D-A594 ATTO 594 100 ug $402
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BHA11901415-5 SMC-189D-APC APC 100 ug $401
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BHA11901415-6 SMC-189D-BI Biotin 100 ug $370
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BHA11901415-7 SMC-189D-FITC FITC 100 ug $370
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BHA11901415-8 SMC-189D-HRP HRP 100 ug $389
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BHA11901415-9 SMC-189D-PCP PerCP 100 ug $401
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BHA11901415-10 SMC-189D-RPE RPE 100 ug $399
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BHA11901415-11 SMC-189S Unconjugated 12 ug $45
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