Ubiquitin Detection Kit

Mfr.No.	SKT-131-20
Alternative Names	Polyubiquitin B Detection Kit, RPS27A Detection Kit, UBA52 Detection Kit, UBB Detection Kit, UBC Detection Kit, ubiquitin B Detection Kit, Polyubiquitin B Detection Kit ; RPS27A Detection Kit ; UBA52 Detection Kit ; UBB Detection Kit ; UBC Detection Kit ; ubiquitin B Detection Kit
Categories	Detection Kits
Description	Capture and detection of ubiquitinated proteins
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	300
Reactivity	Species independent
Scientific Background	The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, transcription, signal transduction, endocytosis, apoptosis and degradation by the proteasome, autophagy and lysosome systems. As such ubiquitin signalling and the processes it mediates are essential for the normal functioning of cells and its dysfunction has been implicated in wide range of diseases including cancer, neurodegeneration, cardiovascular and metabolic disorders (1,2). The type of ubiquitin modification, (monoubiquitin, multiubiquitin, polyubiquitin), substrate protein lysine residue(s) modified and, in the case of polyubiquitination, the chain length and lysine linkage type control the function and fate of ubiquitinated proteins. In addition all ubiquitin mediated pathways also utilise speciﬁc ubiquitin receptors to facilitate their regulation (3,4). Ubiquitination is achieved through three enzymatic steps. In an ATP-dependent process, the ubiquitin E1 activating enzyme catalyses the formation of a reactive thioester bond with ubiquitin, followed by its subsequent transfer to the active site cysteine of a ubiquitin E2 carrier protein. The selectivity of the ubiquitin cascade for a particular substrate protein relies on the interaction between the E2 conjugating enzyme (of which a cell contains relatively few) and an ubiquitin E3 ligase , of which over 600 have been identified to date. The specific E2-E3 pair required for ubiquitination of a particular substrate protein in vivo may also control the type, point and length / linkage (polyubiquitin) of the ubiquitin modification (5).
Size	400µl ; 20 tubes ; 20 tubes ; 25µl
Adr Code	Non-hazardous
Assay Overview	The Ubiquitin Detection kit facilitates the fast, effective capture and detection of ubiquitinated proteins from biological samples. The kit utilises a high capacity, high specificity ubiquitin binding matrix together with an easy-to-use purification system for less ‘hands on time’ and superior performance. Allows purification of mono- and poly-ubiquitinated proteins, independent of chain linkage or length, but not free ubiquitin. Highly adaptable, compatible with samples from a wide range of species and with a broad range of lysis buffers. Analysis by Western blotting or proteomic methods enables identification and assessment of ubiquitinated proteins of interest. Kit contains sufficient ubiquitin matrix to perform up to 20 assays.
Assay Type	Binding Matrix/Quantitative WB (Western Blot)
Assay Type Abbreviation	Purification
Component Number	SKC-131A ; SKC-131B ; SKC-131C ; SKC-131D
Country Of Origin	Canada
Incubation Time	1 hour
Item	StressXpress® ubiquitin matrix (50% slurry) ; StressXpress® columns ; StressXpress® collection tubes ; Ubiquitin antibody (FK2) (HRP)
Method Type	Cell Lysates, Tissue
Number Of Samples	20 samples
Platform	Spin Column
Species Reactivity Abbreviations	ALL
Species Reactivity Medium	Species Independent
Trademark	StressXpress®

Target	Ubiquitin
Research Area	Cell Signaling Post-Translational Modifications Ubiquitination Epigenetics Cancer Apoptosis Autophagy Neuroscience Neurodegeneration Cardiovascular System
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Spin Column
Utility: Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples.

kit-overview:

Component No.	Item	Quantity / Size
SKC-131A	StressXpress® ubiquitin matrix (50% slurry)	400 µl
SKC-131B	StressXpress® columns	20 tubes
SKC-131C	StressXpress® collection tubes	20 tubes
SKC-131D	Ubiquitin monoclonal antibody (FK2) (HRP)	25 µl

Incubation Time: 1 hour
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 300
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples.

Reference	1. Fulda, S., Rajalingam, K. & Dikic, I. EMBO molecular medicine 4, 545–56 (2012). 2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21–30 (2013). 3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291–322 (2012). 4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203–229 (2012). 5. Spasser, L. & Brik, A. Angewandte Chemie (International ed. in English) 51, 6840–62 (2012).

Other Resources 1	Kit Booklet
Other Resources 2	MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHE11900025	SKT-131-20	20 assays	$434		Add to Cart

Ubiquitin Detection Kit

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