Mycoplasma Pro PCR Detection Kit is an improved version of our best-selling kit (Cat. No. G238) which now includes:
a modified Pro Primer Mix and more stringent cycling conditions to include the detection of additional Mycoplasma and Acholeplasma strains such as A. laidlawii
an inteal PCR control that helps confidently distinguish between a genuine negative and a false negative result due to technical errors or PCR inhibition.
Timely detection of Mycoplasmas in cell cultures is recommended to deter wide-spread contamination and save on the costly efforts of elimination. This kit includes a PCR MasterMix containing gel loading dye for added convenience.
Kit Features:
No DNA isolation needed: Add cell culture media sample directly to PCR reaction
Rapid and Comprehensive: Detect 300+ Mycoplasma and Acholeplasma strains in less than 2 hours
Highly Sensitive and Specific: Demonstrated the highest true positive and lowest false positive rates compared to competing kits
Easy-to-use: Includes ready-to-use primer mix, positive control and an inteal PCR control
Out-performs Sigma and Lonza's kits
Combine with our bestselling Mycoplasma Elimination Cocktail (Cat. No. G398) to eliminate 70+ species of Mycoplasma
Kit Contents
Product Components
Quantity
Nuclease-Free H2O
1.0 ml
BlasTaq™ 2X PCR MasterMix
1.25 ml
Pro Primer Mix
100 μl
Positive Control
250 μl
Notes
Why test your cells for Mycoplasma?
Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:
proliferation
metabolism
gene synthesis & processing
adhesion properties
and more
Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting.
Package Size
100 reactions
Storage
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.