MycAway^TM Plus-Color One-Step Mycoplasma Detection Kit

Mfr.No.

40612ES25, 40612ES60

Description

The MycAway™ Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and thus will not affect the judgment of the results.

The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.

MycAway™ Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supeatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method.

Components

Components No.	Name	40612ES25 （25 T)	40612ES60 (100 T)
40612-A	MycAway™ -Color Lamp	425 μL	4 × 425 μL
40612-B	MycAway™-Color Lamp Primer	50 μL	4 × 50 μL
40612-C	Positive Control	10 μL	4 × 10 μL
40612-D	Mineral oil	500 μL	4 × 500 μL

Features

The one-step operation results in less than 60 minutes
Use a water bath or PCR instrument to test
22 mycoplasma species were accurately detected by the kit, including 8 common species
Results just identified by the visual inspection

Reference	[1] Shi N, Yang Q, Zhang H, et al. Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. Nat Biomed Eng. 2022;6(2):195-206. doi:10.1038/s41551-021-00774-1(IF:25.671) [2] Zhang S, Yu F, Che A, et al. Neuroendocrine Regulation of Stress-Induced T Cell Dysfunction during Lung Cancer Immunosurveillance via the Kisspeptin/GPR54 Signaling Pathway. Adv Sci (Weinh). 2022;9(13):e2104132. doi:10.1002/advs.202104132(IF:16.806) [3] Li Y, Xue B, Zhang M, et al. Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency. Genome Biol. 2021;22(1):206. Published 2021 Jul 12. doi:10.1186/s13059-021-02424-w(IF:13.583) [4] Wu L, Xu Y, Zhao H, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12(2):842-858. Published 2022 Jan 1. doi:10.7150/thno.66575(IF:11.556) [5] Tan B, Shi X, Zhang J, et al. Inhibition of Rspo-Lgr4 Facilitates Checkpoint Blockade Therapy by Switching Macrophage Polarization. Cancer Res. 2018;78(17):4929-4942. doi:10.1158/0008-5472.CAN-18-0152(IF:9.130) [6] Yan G, Zhao H, Zhang Q, et al. A RIPK3-PGE₂ Circuit Mediates Myeloid-Derived Suppressor Cell-Potentiated Colorectal Carcinogenesis. Cancer Res. 2018;78(19):5586-5599. doi:10.1158/0008-5472.CAN-17-3962(IF:9.130) [7] Gu Z, Shi C, Li J, et al. Palbociclib-based high-throughput combination drug screening identifies synergistic therapeutic options in HPV-negative head and neck squamous cell carcinoma. BMC Med. 2022;20(1):175. Published 2022 May 12. doi:10.1186/s12916-022-02373-6(IF:8.775) [8] Wu L, Zhang X, Zheng L, et al. RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis. Cancer Immunol Res. 2020;8(5):710-721. doi:10.1158/2326-6066.CIR-19-0261(IF:8.728) [9] Qin J, Zhang X, Tan B, et al. Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer. Cancer Immunol Res. 2020;8(11):1426-1439. doi:10.1158/2326-6066.CIR-20-0123(IF:8.728) [10] Cao M, Huang W, Chen Y, et al. Chronic restraint stress promotes the mobilization and recruitment of myeloid-derived suppressor cells through β-adrenergic-activated CXCL5-CXCR2-Erk signaling cascades. Int J Cancer. 2021;149(2):460-472. doi:10.1002/ijc.33552(IF:7.396) [11] Yao R, Alkhawtani AYF, Chen R, Luan J, Xu M. Rapid and efficient in vivo angiogenesis directed by electro-assisted bioprinting of alginate/collagen microspheres with human umbilical vein endothelial cell coating layer [published correction appears in Int J Bioprint. 2020 Sep 17;6(4):309]. Int J Bioprint. 2019;5(2.1):194. Published 2019 Jun 24. doi:10.18063/ijb.v5i2.1.194(IF:6.638) [12] Liu L, Deng Y, Zheng Z, et al. Hsp90 Inhibitor STA9090 Sensitizes Hepatocellular Carcinoma to Hyperthermia-Induced DNA Damage by Suppressing DNA-PKcs Protein Stability and mRNA Transcription. Mol Cancer Ther. 2021;20(10):1880-1892. doi:10.1158/1535-7163.MCT-21-0215(IF:6.261) [13] Wu S, Yang X, Tang W, et al. Chemotherapeutic Risk lncRNA-PVT1 SNP Sensitizes Metastatic Colorectal Cancer to FOLFOX Regimen. Front Oncol. 2022;12:808889. Published 2022 Mar 31. doi:10.3389/fonc.2022.808889(IF:6.244) [14] Wu Q, Xuan YF, Su AL, Bao XB, Miao ZH, Wang YQ. TNKS inhibitors potentiate proliferative inhibition of BET inhibitors via reducing β-Catenin in colorectal cancer cells. Am J Cancer Res. 2022;12(3):1069-1087. Published 2022 Mar 15. (IF:6.166) [15] Wu L, Zhao KQ, Wang W, et al. Nuclear receptor coactivator 6 promotes HTR-8/SVneo cell invasion and migration by activating NF-κB-mediated MMP9 transcription. Cell Prolif. 2020;53(9):e12876. doi:10.1111/cpr.12876(IF:5.753) [16] Wei Z, Wang Y, Peng J, et al. CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling. Cell Death Discov. 2022;8(1):285. Published 2022 Jun 11. doi:10.1038/s41420-022-01066-6(IF:5.241) [17] Chen W, Weng Z, Xie Z, et al. Sequencing of methylase-accessible regions in integral circular extrachromosomal DNA reveals differences in chromatin structure. Epigenetics Chromatin. 2021;14(1):40. Published 2021 Aug 23. doi:10.1186/s13072-021-00416-5(IF:4.954) [18] Wu Z, Zheng M, Zhang Y, et al. Hsa_circ_0043278 functions as competitive endogenous RNA to enhance glioblastoma multiforme progression by sponging miR-638. Aging (Albany NY). 2020;12(21):21114-21128. doi:10.18632/aging.103603(IF:4.831) [19] Wang L, Zhou Y, Cao C, et al. The exon 12-containing LHX6 isoforms promote cervical cancer cell proliferation by regulating the MAPK signaling pathway [published online ahead of print, 2022 Apr 5]. Cancer Med. 2022;10.1002/cam4.4734. doi:10.1002/cam4.4734(IF:4.452) [20] Zhang Q, Yan G, Lei J, et al. The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer. Mol Med. 2020;26(1):39. Published 2020 May 6. doi:10.1186/s10020-020-00174-2(IF:4.096) [21] Hu J, Wu Q, Wang Z, et al. Inhibition of CACNA1H attenuates doxorubicin-induced acute cardiotoxicity by affecting endoplasmic reticulum stress. Biomed Pharmacother. 2019;120:109475. doi:10.1016/j.biopha.2019.109475(IF:3.743) [22] Wang T, Lin F, Sun X, et al. HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation. Cancer Cell Int. 2019;19:3. Published 2019 Jan 3. doi:10.1186/s12935-018-0717-6(IF:3.439) [23] Meng LL, Wang JL, Xu SP, et al. Low serum gastrin associated with ER⁺ breast cancer development via inactivation of CCKBR/ERK/P65 signaling. BMC Cancer. 2018;18(1):824. Published 2018 Aug 16. doi:10.1186/s12885-018-4717-7(IF:3.288) [24] Ma J, Liu X, Liu P, et al. Identification of a new p53 responsive element in the promoter region of anillin. Int J Mol Med. 2020;45(5):1563-1570. doi:10.3892/ijmm.2020.4527(IF:3.098) [25] Chen L, Chen L, Wan L, et al. Matrine improves skeletal muscle atrophy by inhibiting E3 ubiquitin ligases and activating the Akt/mTOR/FoxO3α signaling pathway in C2C12 myotubes and mice. Oncol Rep. 2019;42(2):479-494. doi:10.3892/or.2019.7205(IF:3.041) [26] Men XM, Xu ZW, Tao X, Deng B, Qi KK. FNDC5 expression closely correlates with muscle fiber types in porcine longissimus dorsi muscle and regulates myosin heavy chains (MyHCs) mRNA expression in C2C12 cells. PeerJ. 2021;9:e11065. Published 2021 Apr 19. doi:10.7717/peerj.11065(IF:2.984) [27] Zhao YQ, Wu T, Wang LF, et al. Targeting MUC1-C reverses the cisplatin resistance of esophageal squamous cell carcinoma in vitro and in vivo. Transl Cancer Res. 2021;10(2):645-655. doi:10.21037/tcr-20-2495(IF:1.241)

Storage	Stored at -15℃ ~ -25℃ for 18 months. Please keep away from the light.
Shipping	All the components are shipped with dry ice.

Subculture Protocol	40612_Manual_HB221128_EN.pdf

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHM20800015-1	40612ES25	25 T	$80		Add to Cart
BHM20800015-2	40612ES60	100 T	$300		Add to Cart

MycAway^TM Plus-Color One-Step Mycoplasma Detection Kit

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