DMEM:Ham's F12, basic

Mfr.No.	820400a
Description	The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. Furthermore, it is an excellent basic medium for the serum free cell culture. pH = 7.2 +/- 0.02 at 20-25°C. Each lot of DMEM:Ham’s F12 (CLS designation MG-40) has been tested for sterility and absence of Mycoplasma and Bacteria. The manufacturing process and documentation are performed according to ISO9001:2008 based on GLP under strict quality control.
Maintenance	Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and warm it up inside a separate device.
Short Description	DMEM:Hams F12 (1:1), basic supplemented with L-glutamine and HEPES Addition of serum or other supplementation required
Glutamine	L-glutamine