CRISPR/spCas9 nuclease AAV AAV (AAV/DJ-CMV-spCas9)

Mfr.No.	7120
Description	Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. This synthetic serotype has better infection efficiency in most cultured cells than wild type AAV serotype 1-8, and it also has superior liver & neuronal tropism for in vivo infection. This is an AAV-DJ expressing spCas9 nuclease under a short CMV7 promoter. It can be used along with guided RNA for targeted genome-editing. We can also package this spCas9 into other serotypes of AAV upon request.
Categories	AAV, Over-Expression, CRISPR/Cas9
Product Type	Pre-Made
Virus Type	AAV
Function	CRISPR/Cas9
Promoter	CMV7 (ubiquitous)
Storage Buffer	PBS/5% Glycerol
Viral Backbone	Recombinant AAV
Shelf Life	12 Months
HS Code	3822190000
UNSPSC Code	41106621
Insert Gene	spCas9
Serotype	AAV2//DJ
Availability	Ready to Ship
Titer	1x10^13 GC/ml
ITRs	AAV2

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SKU	Supplier No.	Size	Your price	Quantity
BHV21600524	7120	50 µL	$495		Add to Cart