Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.This adenovirus expresses both Cre recombinase and GFP. The GFP is in an IRES expression cassette. Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
Categories
Adenovirus, Over-Expression, Recombinase
Product Type
Pre-Made
Virus Type
Adenovirus
Function
Reporter/Tag
Promoter
CMV (ubiquitous)
Reporter
eGFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Shelf Life
12 Months
HS Code
3822190000
UNSPSC Code
41106621
Insert Gene
Cre-IRES-GFP
Availability
Ready to Ship
Titer
1x10^10 PFU/ml
Reporter
eGFP
Storage
-80C
Shipping
Dry Ice
Reviews of Cre Recombinase Adenovirus (Ad-Cre-IRES-GFP)