This is a quick and easy spin column method for the isolation of genomic DNA from various animal tissues or cells. No phenol, chloroform, or other hazardous organic solutions are required. Proteins are degraded by Proteinase K, DNA is bound to the silica-based filter in the spin column, impurities are washed away, and the purified gDNA is eluted from the binding matrix of the column. The isolated genomic DNA is of excellent quality for PCR, restriction digestion, and other downstream applications. The kit contains all necessary tubes for the complete experiment in isolation of genomic DNA
Equipment Required
Microcentrifuge
Pipettes
Features
Versatile, able to isolate gDNA from tissues, cultured cells, paraffin tissue or rodent tails.
Simple, fast and efficient.
High yield and reproducible.
No phenol chloroform extraction or ethanol precipitation required.
High capacity - up to 10 μg of DNA per column.
Storage
All components (except Proteinase K) can be stored at room temperature. Proteinase K should be kept at 4ºC for short-term or -20ºC for long-term storage. Kit components are stable for12 months at room temperature after received. For maximum stability, sto