MDA-MB-157 Cells

Description
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Mfr.No.	305280
Organism	Human
Disease	Carcinoma
Description	The MDA-MB-157 cell line is derived from a human breast carcinoma, specifically from a pleural effusion of a metastatic breast cancer patient. This cell line is extensively used in breast cancer research, particularly for studying the biology of triple-negative breast cancer (TNBC), a subtype that lacks expression of estrogen receptor (ER), progesterone receptor (PR), and HER2/neu. MDA-MB-157 cells provide a valuable model for investigating the molecular mechanisms driving TNBC, as well as for testing potential therapeutic agents targeting this aggressive form of breast cancer. MDA-MB-157 cells exhibit an epithelial morphology and are characterized by their high metastatic potential. They express markers typical of basal-like breast cancer, including cytokeratins 5/6 and epidermal growth factor receptor (EGFR). Researchers utilize MDA-MB-157 cells to explore key signaling pathways involved in TNBC progression, such as the PI3K/Akt, MAPK, and Notch pathways. These cells are also employed in drug screening assays to evaluate the efficacy of chemotherapeutic agents, targeted therapies, and combination treatments. Additionally, MDA-MB-157 cells are used to study the mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the MDA-MB-157 cell line in triple-negative breast cancer research underscores its importance in advancing our understanding of this challenging subtype of breast cancer and in developing more effective therapeutic approaches for TNBC patients.
Synonyms	MDA-MB157, MDAMB157, MDA-157, MDA157, MB 157, MB157, MD Anderson-Metastatic Breast-157
Gender	Female
Age	44 years
Ethnicity	African American
Morphology	Epithelial
Growth Properties	Adherent
Tissue	Breast
Biosafety level	1
Citation	MDA-MB-157 (Cytion catalog number 305280)
Tumorigenic	Yes, in nude mice and in immunosuppressed BALB/c mice
Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Medium Supplements	Supplement the medium with 20% FBS + Insulin (5 microgram/ml)
Freeze Medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

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This cell line is available in the U.S. For non-profit users, please sign and return the Supply Agreement (see Documentation) to orders@biohippo.com when placing an order. For commercial users, please complete the CLEAR Form (see Documentation) as additional usage fee may apply based on the given intended usage. For further details, please contact us at orders@biohippo.com.

SKU	Supplier No.	Size	Your price	Quantity
BHC11101444	305280	1 cryovial	$375		Add to Cart

Datasheet
Safety data sheet
CLEAR-Commercial Customers Form
Supply-agreement (non-profit)

MDA-MB-157 Cells

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