AC16 Cardiomyocyte Cell Line

Description
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Mfr.No.	305215
Cell Type	Cardiomyocyte
Organism	Human
Description	The AC16 cell line, derived from human ventricular cells fused with SV40-transformed, showcases characteristics typical of cardiomyocytes, including the expression of transcription factors such as GATA4, MYCD, NFATc4, and contractile proteins like alpha- and beta-myosin heavy chain. AC16 cells also express gap junction proteins connexin-43 and connexin-40, with functional gap junctions confirmed by dye-coupling studies, underscoring their utility in cardiomyocyte research. When the SV40 oncogene is silenced, AC16 transitions towards a more differentiated state, marked by the expression of BMP2, indicative of cardiac differentiation and developmental regulation. In general, scientists employ various techniques, including stem cell differentiation, animal models, molecular analysis, and biomarker discovery, to advance knowledge and potential therapies for heart-related conditions. The involvement of mitogen and senescence pathways, along with thymidine kinase induction, further elucidates the complex nature of human cardiomyocytes and their response to pathological conditions. The AC16 human cardiomyocyte cell line's ability to mimic the behavior of mature cardiomyocytes makes it a valuable model for cardiac research. It closely resembles the genetic makeup of primary cardiomyocytes, allowing for studies on cardiac development, pathology, and the implications of histone loss in vitro, however, the cardiomyocyte behavior and genetic complexity might not fully match that of primary or stem cell-derived cardiomyocytes. In the context of toxicology and cardiovascular disease research, AC16 cells serve as a vital tool for understanding cardiomyocyte development, inflammation, injury, regeneration, and toxicological effects. The unique properties of the AC16 human cardiomyocyte cell line, including its response to developmental cues and the ability to simulate the physiological conditions of human cardiomyocytes, make it an indispensable asset in the quest to unravel the mysteries of heart diseases and devise novel therapeutic interventions.
Synonyms	Human hybrid cardiomyocyte
Ethnicity	Caucasian
Morphology	Epithelial
Growth Properties	Adherent
Tissue	Heart, Ventricle
Biosafety level	1
Citation	AC16 (Cytion catalog number 305215)
Applications Range	Research in toxicology and cardiovascular disease focuses on understanding cardiomyocyte development, inflammation, injury, regeneration, and toxicological effects. Scientists use various techniques, including stem cell differentiation, animal models, molecular analysis, and biomarker discovery, to advance knowledge and potential therapies for heart-related conditions.
Viruses	Transformed by the SV40 large T-antigen
Culture Medium	DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Medium Supplements	Supplement the medium with 12.5% FBS. Add 0.9 mM L-Glutamine to achieve a final concentration of 2.5 mM L-Glutamine.
Passaging Solution	Accutase
Freeze Medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR Profile	Amelogenin: x,x CSF1PO: 9,11,12 D13S317: 12,13 D16S539: 11,13 D5S818: 9,11 D7S820: 10,11,12 TH01: 7,8,9.3 TPOX: 11 vWA: 16,18 D3S1358: 17,18 D21S11: 32.2,33.2 D18S51: 12,17 Penta E: 7,8,16 Penta D: 2.2,9 D8S1179: 12,14 FGA: 21,25

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This cell line is available in the U.S. For non-profit users, please sign and return the Supply Agreement to orders@biohippo.com when placing an order. For commercial users, please complete the CLEAR Form as additional usage fee may apply based on the given intended usage. For further details, please contact us at orders@biohippo.com.

SKU	Supplier No.	Your price	Quantity
BHC11101343	305215	$540		Add to Cart

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AC16 Cardiomyocyte Cell Line

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