Mouse Anti-Human CENP-A Monoclonal IgG1

Mfr.No.	SMC-202, SMC-202D, SMC-202D-A390, SMC-202D-A488, SMC-202D-A594, SMC-202D-APC, SMC-202D-BI, SMC-202D-FITC, SMC-202D-HRP, SMC-202D-PCP, SMC-202D-RPE, SMC-202S
Immunogen Species	Human
Alternative Names	CENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Chromosome, Centromere, Kinetochore
Certificate Of Analysis	1 ug/ml of SMC-202 was sufficient for detection of CENPA in 20 ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human CENP-A Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide corresponding to a portion of human CENP-A, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide corresponding to a portion of human CENP-A
Isotype	IgG1
Launch Date	2012/04/23
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Antigen	CENPA
Swissprot	P49450
Accession Number	NP_001035891.1
Gene Id	1058
Target	CENPA
Clone No	5A7-2E11
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~18kDa. Recognizes Human CENP-A.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188. 3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223. 4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901605-1	SMC-202D	Unconjugated	100 ug	$312	Add to Cart
BHA11901605-2	SMC-202D-A390	ATTO 390	100 ug	$367	Add to Cart
BHA11901605-3	SMC-202D-A488	ATTO 488	100 ug	$366	Add to Cart
BHA11901605-4	SMC-202D-A594	ATTO 594	100 ug	$366	Add to Cart
BHA11901605-5	SMC-202D-APC	APC	100 ug	$365	Add to Cart
BHA11901605-6	SMC-202D-BI	Biotin	100 ug	$361	Add to Cart
BHA11901605-7	SMC-202D-FITC	FITC	100 ug	$357	Add to Cart
BHA11901605-8	SMC-202D-HRP	HRP	100 ug	$352	Add to Cart
BHA11901605-9	SMC-202D-PCP	PerCP	100 ug	$365	Add to Cart
BHA11901605-10	SMC-202D-RPE	RPE	100 ug	$362	Add to Cart
BHA11901605-11	SMC-202S	Unconjugated	12 ug	$45	Add to Cart

CENP-A Antibody, Clone 5A7-2E11 (SMC-202)