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Home / Cells / Cell Lines

TCR/B2M Knockout Jurkat Cell Line

SKU : BHC18200155

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BPS Bioscience

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Mfr.No.	78552
Description	This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRalpha/beta chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (Biohippo #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (Biohippo #79796)
Background	The T Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR triggers a signaling cascade that leads to the activation of transcription factors involved in the upregulation and secretion of cytokines, T cell proliferation, and cell differentiation into effector and memory cells. TCR-activated transcription factors include AP-1 (Activator Protein 1), NF-kappaB (Nuclear Factor Kappa-light-chain-enhancer of activated B cells) and NFAT (Nuclear Factor of Activated T-cells).Beta-2-Microglobulin is a required component of Major Histocompatibility Complex (MHC) class 1 molecules, which present peptide fragments from within the cell to cytotoxic T cells as part of the adaptive immune system. B2M plays an essential role both in governing MHC class I molecule stability and in promoting antigen binding by presenting the antigen to CD3/TCR complex of CD8+ T cells.Knockout of both TCR and B2M will support the manufacture of universal CAR-T cells. Knockout of TCR or B2M prevents the elimination of allogeneic T cells that express foreign HLA-I molecules, thereby enabling the generation of CAR-T cells from allogeneic healthy donors T cells in vivo.
Application	Useful for the development of improved universal CAR-T or other effector cells.
Host Cell	Jurkat (clone E6-1), human T lymphoblast, suspension
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email orders@biohippo.com. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200155	78552	2 vials	$9509.50		Add to Cart

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