The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.
Mycoplasma testing: The cell line has been screened using the MycoAlert Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine stimulating the production of granulocytes and monocytes from bone marrow precursors. GM-CSF had pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes.
Application
1. Screen for activators or inhibitors of the STAT5 signaling pathway. 2. Screen for the neutralization antibody of GM-CSF.
Host Cell
U937
Species
Human
Synonyms
Signal Transducer And Activator Of Transcription 5A cell line, MGF cells, U937 Reporter
Shipping Temperature
-80°C (dry ice)
Note
Visit Biohippobioscience.com/license for the label license and other key information about this product. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.