Tumorigenecity: Yes, in nude mice and also in hamster cheekIsoenzyme: Me-2, 2; PGM3, 1-2;PGM1, 1;ES D, 2;AK1, 1;GLO-1, 1-2;G6PD, B.Histopathology: Neuroblastoma, metastasis to supra-orbital areaSubculture: Remove medium, rinse with fresh 0.25 % Trypsin solution, remove trypsin and let the culture sit at room temperature(or at 37'C) until the cells detach(about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days.Split ratio: Media change: Reverse transcritase: Production.