Prostaglandin I2 Receptor cell line

Mfr.No.	AG-10200-247
Description	cAMP assay for Gs-coupled human Prostaglandin I2 Receptor (PTGIR). HEK293-CNG cells (AG-10200-200) without transfected Prostaglandin I2 Receptor are used as a negative control.
Gene Name	Human Prostaglandin I2 Receptor (PTGIR)
Cell Origin	Human
Culture Medium	Growth medium: 90% DMEM, 10% FBS, 250 µg/ml G418 and 1 µg/ml puromycin Freezing medium: 10% DMSO, 90% complete medium

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Figure 1. Response of ACTOne PTGIR cell line to Iloprost. ACTOne PTGIR cells were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of Iloprost. Ratios of the two readings (F/F0) are plotted in the figure. EC50 = 5.0 pM in the presence of PDE inhibitor Ro20-1724. Parental cells dont respond to Iloprost (Data not shown)

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Prostaglandin I2 Receptor cell line

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