Mouse Skeletal Muscle Myoblasts (MSkMM)

Product Description

Myoblast is a type of progenitor cells that differentiates to give rise to muscle cells ⁽¹⁾. They are the main effector cells in the myogenesis by fusing with each other to injured myofibers leading to the formation of new myofibers or regenerations of skeletal muscle in adults ⁽²⁾. Many steps of myogenesis can be recapitulated through in vitro differentiation of myoblasts into myotubes. Primary myoblasts have been suggested as the most physiologically relevant model for studying myogenesis in vitro ⁽³⁾.

MSkMM from iXCells Biotechnologies are derived from mouse skeletal satellite cells isolated from mouse gastrocnemius (GA) and tibialis anterior (TA) muscles of both hind limbs by culturing them on matrigel-coated plastic dishes (Figure 1). MSkMM are cryopreserved at passage one and delivered frozen. Each vial contains ?5 x 10⁵ cells/vial. MSkMM are characterized by immunofluorescence with antibodies specific to MyoD and Desmin (Figure 2). MSkMM can be in vitro differentiated into myotube using Mouse Skeletal Muscle Myoblast Differentiation Medium (Cat#MD-0065). MSkMM are guaranteed to further expand two passages using the culture medium provided by iXCells. These cells are negative for mycoplasma, bacteria, yeast and fungi.

Figure 1.  Mouse Skeletal Muscle Myoblasts (MSkMM) were recovered and seeded at 1X10⁴/cm² in Mouse Skeletal Muscle Myoblast Growth Medium (Cat# MD-0064). Phase contrast images were taken at the indicated time post recovery.

Figure 2.  Mouse Skeletal Muscle Myoblasts (MSkMM) were fixed at DIV1 and ICC staining was performed using antibodies against MyoD and Desmin, separately.

Figure 3. Mouse Skeletal Muscle Myoblasts (MSkMM) were cultured in Mouse Skeletal Muscle Myoblast Growth Medium (Cat# MD-Figure 3. Mouse Skeletal Muscle Myoblasts (MSkMM) were cultured in Mouse Skeletal Muscle Myoblast Growth Medium (Cat# MD-0064) until the cells reach 70-80% confluency. The growth medium was replaced with Mouse Skeletal Muscle Myoblast DifferentiationMedium (Cat# MD-0065) for 48 hours. The phase contrast image was shown indicating the formation of myotubes.

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References

(1) R L Perry, M A Rudnick. Molecular mechanisms regulating myogenic determination and differentiation. Front Biosci. 2000, 5: D750-67

(2) Roong Zhao, Alistair J Watt, Michele A Battle, Jixuan Li, Benjamin J Bondow, Stephen A Duncan. Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice. Dev Biol. 2008, 317(2): 614–619.

(3)  Brendan Evano, Shahragim Tajbakhsh. Satellite cells and the muscle stem cell niche. Physiol Rev. 2013, 93(1):23-67.