Rad9; a checkpoint gene; plays a key role in the regulation of cellular response to DNA damage and the maintenance of genomic integrity. Mouse Rad9 -/- Embryonic Stem Cells were developed using a Cre/Loxp conditional knockout system. This cell line is suitable for research regarding the functions of Rad9; including the effect of loss of function in tumorigenesis.
Biosafety Level
BioSafety Level II
Growth Properties
Adherent
Quantity
106 cells/vial
Morphology
Polygonal
Species
Mouse (M. musculus)
Propagation
Use of Gelatin Coating Solution (0.1%) (TM063) is required for cell adhesion to the culture vessels. Grow cells in gelatin-coated culture vessels with the following conditions. The base medium for this cell line is Knockout DMEM medium (Gibco).To make the complete growth medium, add fetal bovine serum (TM999) to a final concentration of 15%, penicillin-streptomycin solution (a href="https://www.abmgood.com/penicillin-streptomycin-solution-g255.html#G255">G255) to a final concentration of 5%, 2mM L-glutamine (a href="https://www.abmgood.comhttps://www.abmgood.com/l-glutamine-g275.html#G275#G275">G275), 10^(-4)M 2-Mercaptoethanol, 10^3 units/mL of ESGRO (Millipore), and 0.1mM non-essential amino acids solution (Gibco). Change media every 2-3 days. Carbon dioxide (CO2): 5%, Temperature: 33.0°C. * Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Markers
Mrad9 -/- ES cells were transfected with pZeoSV2-Mrad9 and pZeoSV2-HRAD9 and then challenged with zeocin