The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 0.1 mM Non-Essential Amino Acids, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Source Organ
Reproductive
Shipping
Dry Ice
Storage
-180°C
Reviews of Mouse Embryonic Fibroblast Feeder Cells