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Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP)

SKU : BHC10902195

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0204
Description	This cell line is an immortalized neutrophil progenitor cell line dervied from?57Bl/6 Cas9-GFP transgenic mice. Cells express GFP at low concentrations that are detectable by flow cytometry (note: it can be impossible to see GFP expression by fluorescent microscopy).?B8e-C57-Cas9GFP cells are transduced with ER-HoxB8. Cells are capable of differentiation into mature neutrophils.?dditional cell lines within this panel include: Cat. T0200 - Immortalized Myeloid Granulocyte Cell Line (ECoM-G) Cat.?0201 - Immortalized Myeloid Monocyte Cell Line (ECoM-M) Cat.?0202 - Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO) Cat.?0203 - Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO) Cat.?0204 - Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP) Cat.?0205 - Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP) Cat.?0207 - Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57) Cat.?0209 - Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	University of California, San Diego
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties	Suspension
Growth Conditions	PriCoat?T25 Flasks (G299) are recommended for optimal cell culture;?assage to new plastic with fresh media at least every 4 days.?he base medium for this cell line is PriGrow II (TM002) supplemented with 10% FBS + 100 ng/ml Stem Cell Factor (SCF) + 0.5 ?M ?estradiol (0.5 ?M) + 1% Penicillin/Streptomycin (G255),37.0?, 5% CO?.?-estradiol is required for cell maintenance but must be completely removed for cell differentiation.?ote: cells are sensitive to freeze thaw conditions. Do not thaw cells completely; immediately spin down cells and re-suspend in complete growth medium supplemented with 15% FBS to help cells recover for the first 24 hours.?or information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.
Tissue	Bone Marrow
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Mouse (M. musculus)
Cryopreservation	10% DMSO + 50% FBS + 40% Complete Growth Media.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 500xg for 5 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.Note:?ells are sensitive to recovery from freeze thaw conditions. Therefore, significant cell death may be noted in the initial days post-thawing. However, cells will recover. Performing hemi-depletions of the media may aid in recovery during the initial period. Media and culture vessels must be changed every 4 days.
Subculture Protocol	1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10? cells/ml.?br>2. Alternatively, replace complete growth media by centrifuging cells and re-suspending the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10902195	T0204	1x10^6 cells / 1.0 ml	-		Add to Quote

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