JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cells / Cell Lines

Immortalized Mouse Thyrotrope Cells (T?T-1)

SKU : BHC10900847

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Applied Biological Materials (abm) Inc.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	T0800
Description	The Immortalized Mouse Thyrotrope Cells (T?T-1) represent a significant advancement in the study of thyrotrope biology and thyroid hormone regulation. These cells were developed through targeted tumorigenesis in transgenic mice, which involved engineering mice to express an oncogene under the control of the human glycoprotein ?-subunit regulatory region. This innovative approach led to the creation of a clonal cell line that expresses both the ?- and ?-subunits of Thyroid Stimulating Hormone (TSH), which are crucial for maintaining metabolic functions and regulating thyroid hormone levels in the body.One of the key features of the T?T-1 cells is their expression of thyrotrope-specific markers, making them a valuable tool for molecular and biochemical analyses. These cells express thyroid hormone receptors (TRs) ?1 and ?2, as well as their heterodimeric partner, retinoid X receptor-? (RXR?). Functional assays have demonstrated that treatment with triiodothyronine (T3) causes a dose- and time-dependent decrease in TSH ?-subunit mRNA expression, indicating that T?T-1 cells retain the classic endocrine feedback regulation seen in normal thyrotrope cells. This regulation is a defining characteristic that confirms the cells?origin from the pars distalis region of the pituitary gland. Compared to traditional models, T?T-1 cells offer a stable and homogeneous cell population that can be continuously cultured, providing a consistent and reliable model for in-depth studies. These cells are particularly advantageous for mapping the cis- and trans-acting factors involved in thyrotrope-specific and thyroid hormone-regulated gene expression. Overall, these cells are suitable for investigating the molecular mechanisms underlying TSH regulation and thyroid hormone feedback. Their development marks a significant milestone in endocrine research, offering new opportunities for exploring the intricate dynamics of pituitary function and thyroid hormone homeostasis.
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	University of California San Diego
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Region	?-GSU and TSHb
Growth Properties	Lightly adherent, form clusters of polygonal cells
Growth Conditions	Use of 1:10 Matrigel is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.
Quantity	1x106 cells / 1.0 ml
Tissue	Pituitary
Morphology	Polygonal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Mouse (M. musculus)
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Quality Control	1) Genotype analysis; 2)NF-kappaB Activation Assay; 3) Cytokine ELISA Assays; 4) 3(H)-thymidine Cell Proliferation Assay

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 300xg for 3 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media.?br>2. Add fresh complete media and pipette up and down to dissociate adherent cells. Trypsinization is not required.? 3. Plate the cell suspension at a 1:3 to 1:5 dilution once per week.?br>4. Incubate the cells at the recommended conditions.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHC10900847	T0800	1x10^6 cells / 1.0 ml	-		Add to Quote

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.