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Immortalized Human T Cells

SKU : BHC10900698

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0086
Description	The Immortalized Human T Cells are genetically modified to proliferate indefinitely while retaining their functional characteristics, making them an invaluable tool for immunological research and therapeutic development. These cells offer a consistent and reliable model for studying T cell biology, including mechanisms of activation, signaling pathways, and immune responses. Additionally, they are widely used in preclinical studies for developing and testing novel immunotherapies, including CAR-T cell therapies and vaccines. Their ability to be expanded in vitro without senescence allows for large-scale production and in-depth functional assays, facilitating advancements in personalized medicine and immunotherapy research.
Cell Type	Immortalized Cells
Biosafety Level	2
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Donor History	Healthy donor, no other details disclosed
Expression Region	CD3 and HLA-27 (via FACS)
Growth Properties	Suspension, round; cells grow as round clumps
Growth Conditions	T12.5 flasks?re recommended for optimal cell culture.?riGrow X Series Medium (TM0086) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.?Immunocult Human CD3/CD28 T Cell Activator (Stemcell, Cat. 10971) is required for T cell activation upon thawing.?ote: refer to the detailed "Thawing Protocol" and "Subculturing Protocol" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. The post-thaw viability of T0086 can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in T12.5 cell culture flasks. Do not grow cells in culture vessels exceeding a surface area of 25cm2.
Quantity	1x106 cells / 1.0 ml
Tissue	Blood
Morphology	Fibroblast-like
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Population Doubling	4 - 6 days
Species	Human (H. sapiens)
Cryopreservation	Cryopreservation Medium (TM024), or 50% complete growth media, 40% FBS,and 10% DMSO.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media supplemented with 10% FBS. Centrifuge cells at 125xg for 5 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 1ml of the recommended pre-warmed, complete growth media and dispense into a T12.5 culture flask with 3ml of complete growth medium. 5. Add 25? of?mmunocult Human CD3/CD28 T Cell Activator (Stemcell #10971) per ml of total volume (100? for 4ml culture volume).?br />6. Incubate the cells at the recommended conditions and allow cells to grow undisturbed for at least 3 days. Healthy cells will form round clumps.? 7. To feed cells, add fresh complete growth media every 3 days.?br />8. Monitor cell growth and subculture as needed.
Subculture Protocol	Once the culture vessel appears to be 80-90% confluent, subculture cells as per the instructions below.?onfluent cultures may be transferred to T25 culture flasks which can be split to additional T25 flasks. Do not subculture cells to flasks with larger surface area than T25.?. Simply add fresh complete media directly to the culture flask.?R 2. Remove half of the cell suspension and transfer to a new culture vessel; add additional fresh complete media to both cutlure vessels. 3. Incubate the cells at the recommended conditions. 4. Continue to monitor cell growth (cell clumps should continue to grow and become visible in the culture flask).?br>5. If cell growth becomes slow, supplement the culture medium with 20ng/ml IL-2 and incubate cells for 3 days.?br>6. Repeat step 5 if continued slow growth is observed.Note: Do not centrifuge cells during subculture procedures.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900698	T0086	1x10^6 cells / 1.0 ml	-		Add to Quote

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