Prostate cancer research has been hindered by the fact that the cell types involved in the process of malignant transformation have proven difficult to develop into continuously growing cultures. Epithelial cells established in primary culture after radical prostatectomy from a 66-year-old patient were subjected to retroviral infection with human telomerase catalytic subunit (hTERT), thus creating the Immortalized Human Prostate Epithelial Cells (EP156T) - hTERT line. These cells underwent up to 200 population doublings, express genes consistent with a prostate basal epithelial phenotype (cytokeratin 5/6/7/8/14/18, p63, estrogen receptor-beta and androgen receptor) and, like primary cells, differentiate as spheroids with a secretory structure when grown in three-dimensional culture. The generation of this cell line by way of a method that minimizes complex alterations results in a faithful in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact orders@biohippo.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Use of PriCoatTM ?T25 Flasks (G299) or Applied CellExtracellular Matrix (G422) is required for cell adhesion to theculture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the PropagationRequirements below.The base medium for this cell line is MCDB 153 medium (Sigma). To makethe complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)*to a final concentration of 1%, Penicillin/Streptomycin Solution (G255) to a finalconcentration of 1%, MEM Non-essential Amino Acid Solution to a final concentration of 1X, bovine pituitaryextract (Gibco) to a final concentration of 50 mug/mL, insulin (Sigma) to a final concentration of 5 mug/mL,transferrin (Sigma) to a final concentration of 5 mug/mL, EGF (Sigma) to a final concentration of 5 ng/mL,hydrocortisone to a final concentration of 200 nM, triiodothyronine (Sigma) to a final concentration of 10 nM,testosterone (Sigma) to a final concentration of 10 nM and sodium selenite (Sigma) to a final concentration of 5ng/mL.Change media every 2-3 days.Carbon dioxide(CO2): 5%, Temperature: 37.0°C.* Do not useheat-inactivated FBS for cell culture unless specified otherwise.
Quality Control
1) Western blot; 2) qRT-PCR; 3) Three-dimensional cell culture
Reviews of Immortalized Human Prostate Epithelial Cells