Human Umbilical Vein Endothelial Cells (HUVEC)

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis) ⁽¹⁾. They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth ⁽²⁾. HUVECs can be easily made to proliferate in a laboratory setting. They exhibit a cobblestone phenotype when lining vessel walls.

iXCells Biotechnologies provides high quality Human Umbilical Vein Endothelial Cells (HUVEC), which are isolated from human umbilical vein of mix donors, and cryopreserved at P2, with >0.5 million cells in each vial. HUVEC have “cobblestone” morphology and positive staining with vWF/Factor VIII and CD31. HUVEC are one of the mostly used cell types to study endothelial function in vitro, including angiogenesis ⁽¹⁾, signaling pathway under normal and pathological condition such as oxidative stress, hypoxia ⁽²⁾ and inflammation etc. These HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies. Additional expansion may decrease the purity.