cAMP assay for Gi-coupled human glutamate receptor, metabotropic 8 (GRM8).
HEK293-CNG-Slcla3 cells (AG-10200-238) without transfected GRM8 are used as a negative control.
Gene Name
Human Glutamate Receptor, Metabotropic 8 (GRM8)
Cell Origin
Human
Culture Medium
Growth medium: 90% DMEM with Glutamax, 10% FBS, 250 µg/ml G418, 1 µg/ml puromycin and 5 µg/ml blasticidin
Freezing medium: 10% DMSO, 90% complete medium
No Description Available.
Figure 1. Response of ACTOne GRM8 cell line & parental cell line to (R,S)-phosphonophenylglycine. ACTOne GRM8 cells and parental cells (AG-10200-200) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 20 min after the addition of (R,S)-phosphonophenylglycine. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of (R,S)-phosphonophenylglycine in ACTOne GRM8 cell line. EC50 = 828 nM in the presence of PDE inhibitor Ro20-1724 and b-adrenoceptor agonist isoproterenol. B. Parental cells do not respond to (R,S)-phosphonophenylglycine.
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