Mycoplasma testing: This cell line has been screened using the Venor GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Host Species: human
Supplied as Each vial contains 2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Background
RPMI 8226 cells are human B cells isolated from a plasmacytoma/myeloma patient. RPMI 8226 cells constitutively express B-Cell Maturation Antigen (BCMA), and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies, such as Chimeric Antigen Receptor (CAR) T cells. The Firefly Luciferase - RPMI 8226 Recombinant Cell Line makes an excellent target to measure specific killing of CAR-T or NK cells targeting BCMA.
Application
Use as a control for co-culture killing assays.
Host Cell
RPMI 8226
Synonyms
BCMA cell line, CAR T cell line
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact orders@biohippo.com for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid multiple freeze/thaw cycles.
Storage Stability
Store in liquid nitrogen immediately upon receipt.