Dopamine Receptor D2 cell line

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Figure 1. Dose response curve of dopamine in ACTOne DRD2 cell line. ACTOne DRD2 and parental cells (AG-10200-200) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of dopamine. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of Dopamine in ACTOne DRD2 cell line. EC50 = 2.8 nM in the presence of 25 mM of PDE inhibitor Ro20-1724 and 300 nM of b-adrenoceptor agonist isoproterenol. B. Parental cells do not respond to Dopamine.