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Diabetic Mouse Liver Fibroblasts

SKU : BHC16400914

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Cell Biologics, Inc.

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Mfr.No.	MD-6019
Description	Diabetic MouseLiver Fibroblasts fromCell Biologics are isolated from the liver of Diabetic (db/db) mice(8 weeks).Diabetic MouseLiver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	Diabetic Mouse Liver Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	0.5×10⁶cells
General Information	Diabetic Mouse Liver Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics . Repeated freezing and thawing of cells is not recommended.
Tissue	Liver

Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400914	MD-6019	Frozen Vial	$590		Add to Cart

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