Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5). Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: hamster
Supplied as Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (Biohippo #79796)
Background
Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-6 is one of the 27 members of the claudin family.Claudin-6 is widely expressed in various tumors but rarely expressed in healthy adult tissues. This cancer specific expression of claudin-6 makes it a promising target as an antigen for cancer immunotherapy. Several therapeutics that target claudin-6 are currently in development or in clinical trials including bispecific antibodies, monoclonal antibodies, and CAR-T cell therapy products.
Application
Screen and validate antibodies against claudin-6 for drug discovery and research.Screen for compounds that regulate or inhibit claudin-6 signaling in a cellular context.Perform binding assays to screen for potential claudin-6 ligands.
Host Cell
CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Uniprot
P56747
Synonyms
Skullin, CLDN6, UNQ757, PRO1488
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email orders@biohippo.com Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.