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Cas9-Expressing Neuro2A Cell Pool

SKU : BHC18200079

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BPS Bioscience

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Mfr.No.	78087
Description	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Neuro2A cell pool can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. Mycoplasma testing: This cell pool has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: mouse Supplied as Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Application	1. Quickly generating knock-out cell pools or cell lines in Neuro2A cells. 2. Implementing sgRNA screens in Cas9-expressing Neuro2A cells.
Host Cell	Neuro2A
Species	S. pyogenes
Synonyms	CRISPR cell pool, Neuro2A cell pool, Cas9 cell pool, mouse neuroblastoma, Adherent neuroblast cells
Shipping Temperature	-80°C (dry ice)
Note	The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights. License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact orders@biohippo.com for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store in liquid nitrogen.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200079	78087	2 vials	$874.50		Add to Cart

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