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C57BL/6 Mouse Primary Spleen Fibroblasts

SKU : BHC16400542

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Cell Biologics, Inc.

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Mfr.No.	C57-6022
Description	C57BL/6 Mouse Primary Spleen Fibroblasts from Cell Biologics are isolated from the Spleen tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. C57BL/6 Mouse Primary Spleen Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	C57BL/6 Mouse Primary Spleen Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although C57BL/6 Mouse Spleen Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	C57BL/6 Mouse Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. C57BL/6 Mouse Primary Spleen Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Media	Complete Fibroblast Medium w/ Kit 500 ml
Tissue	Spleen
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse
Species details	C57BL/6 Mouse

Applications	Hematopoietic Research, Immunologic Research

Product Use	C57BL/6 Mouse Primary Spleen Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400542	C57-6022	0.5x106 Cells	$470		Add to Cart

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