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BirA-transformed Competent E. coli cells

SKU : BHC18200001

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BPS Bioscience

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Mfr.No.	27462
Description	BirA-transformed Competent E. coli cells are supplied as 10 x 50 ul vials.Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirAexpression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene.These cells are compatible with most cloning vectors and are suitable for expression andbiotinylation of recombinant proteins using AviTag technology. Host Species: E. coli Supplied as 10 x 50 ul vials
Background	Biotin-labeling is commonly used for non-radioactive labeling and purification of proteins and other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) and either avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions of a protein and ligand, exhibiting a dissociation constant (Kd) around 10-15. BirA protein ligase covalently adds biotin to biotin-acceptor peptides containing an AviTag sequence in a highly efficient and specific ATP-dependent reaction.
Application	Useful for specific labeling of proteins/peptides with biotin for downstream applications.
Species	E. coli
Shipping Temperature	-80°C (dry ice)
Note	Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store vials in liquid nitrogen.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200001	27462	10 vials	$539		Add to Cart

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