Adenylate Cyclase Activating Polypeptide 1 Receptor cell line

Mfr.No.	AG-10200-234
Description	cAMP assay for Gs-coupled human Adenylate Cyclase Activating Polypeptide 1 Receptor (ADCYAP1R1). HEK293-CNG cells (AG-10200-200) without transfected Adenylate Cyclase Activating Polypeptide 1 Receptor are used as a negative control.
Gene Name	Human Adenylate Cyclase Activating Polypeptide 1 Receptor (ADCYAP1R1)
Cell Origin	Human
Culture Medium	Growth medium: 90% DMEM, 10% FBS, 250 µg/ml G418 and 1 µg/ml puromycin Freezing medium: 10% DMSO, 90% complete medium

Figure 1. Response of ACTOne ADCYAP1R1 cell line to PACAP. ACTOne ADCYAP1R1 cells were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of PACAP. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of PACAP in ACTOne ADCYAP1R1L cell line. EC50 = 107.5 pM in the absence of PDE inhibitor Ro20-1724. B. Parental cells do not respond to PACAP (data not shown).

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Adenylate Cyclase Activating Polypeptide 1 Receptor cell line

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