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ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line

SKU : BHC18200032

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BPS Bioscience

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Mfr.No.	60541
Description	Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcgammaRIIIa, high affinity (V158) variant and Fcgamma chain. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. Host Species: human
Background	The antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of cell-mediated immune defense whereby an effector cell of the immune system actively lyses a target cell, whose membrane-surface antigens have been bound by specific antibodies. The typical ADCC involves activation of natural killer (NK) cells by antibodies. NK cells express Fc receptors, mostly CD16 or FcgammaRIIIa (CD16a), on its cell surface. These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-gamma and cytotoxic molecules that attack the pathogen-infected target cell. The human FcgammaRIIIa displays a dimorphism in the position of residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158, and the other (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.
Application	Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.
Host Cell	Jurkat
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact orders@biohippo.com for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Storage Stability	Immediately upon receipt, store in liquid nitrogen.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200032	60541	2 vials	$7865		Add to Cart

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