WSU-DLCL Cells

Mfr.No.	T8232
Description	WSU-DLCL cells were derived from a 32 year old female human with multidrug resistant (MDR) histiocytic lymphoma. This cell line displays phenotypic features of a mature B-cell. WSU-DLCL Cells are grown in suspension and form in clumps with large cellular size. The cell line expresses high amounts of P-glycoprotein for multi-drug resistance and chromosomal abnormalities with hyperetripolidy. WSU-DLCL cells are great for research on the MDR gene and its role in chemotherapy resistance.
Cell Type	Tumor Cells
Biosafety Level	2
Depositor	Wayne State University
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Donor History	Female, 32, Olfactory groove meningioma
Expression Region	Multidrug resistance (MDR), high levels of?-glycoprotein
Expression Level	GFP, Kras?¹²?, and reduced expression of PTEN and p53 (PCR)
Growth Properties	Suspension, clumps
Growth Conditions	PriCoat?T25 Flasks (G299)?re recommended for optimal cell culture.?riGrow II (TM002) + 20% heat-inactivated FBS + 2mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.Note: cells are sensitive to post-thaw conditions and take additional time to recover. We recommend supplementing with additional FBS (25-30%) during the first week post-thaw. During the initial growth period (1-2 weeks) centrifuge half the cell suspension and discard supernatant, then combine cells with the leftover cell suspension and transfer to new cutlure vessels. Cells have a tendency to clump in culture. Change media to fresh media every 3 days.
Quantity	1x10? cells / 1.0 ml
Tissue	Blood (Immune)
Morphology	Polygonal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Population Doubling	24 h
Species	Mouse (M. musculus)
Propagation	Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Cryopreservation	Cryopreservation Medium: 40% HI-FBS + 10% DMSO + 50% Complete Growth Media.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. 6. Change media to fresh media every 3 days.
Subculture Protocol	1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10? cells/ml. 2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions. 4. Change media to fresh media every 3 days.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900340	T8232	1x10^6 cells / 1.0 ml	-		Add to Quote

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