The STAT3 Reporter (eGFP)-HEK293 cell line is designed to monitor the STAT3 signal transduction pathway. It contains an eGFP gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the eGFP reporter gene.
Mycoplasma testing: The cell line has been screened using the MycoAlert Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Application
Monitor the STAT3 signaling pathway activity Screen for activators or inhibitors of the STAT3 signaling pathway
Formulation
Thaw Medium 1 (Biohippo #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)Growth Medium 1N (Biohippo #79801): Thaw Medium 1 (Biohippo #60187) plus 0.5 ug/ml of Puromycin (InvivoGen, #ant-pr-1).Assay Medium: Thaw Medium 1 (Biohippo #60187)
Host Cell
HEK293
Synonyms
Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA, IL-6, IL6
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email orders@biohippo.com. Warning: Avoid freeze/thaw cycles. Do not use a -80°C freezer for long term storage.
Storage Stability
Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.