Human Glioblastoma Cell (U87 MG) are isolated from the brain glioblastoma tissue by centrifugal force following enzymatic digestion. RFP-Tagged Human Glioblastoma Cells (RFP-U87MG) are selected from U87MG cells after infected with lentiviruses expressing RFP with Zeocin. The cells are shipped in a frozen vial with more than 5 x 105 cells/vial. Universal Full Growth Medium is recommended for cell culture and these cells have an average minimum population doubling levels ; 20 when cultured following the detailed protocol described below).