Prostaglandin E Receptor 4 cell line

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Figure 1. Response of ACTOne PTGER4 cell line & parental cell line to PGE2. ACTOne PTGER4 cells and parental cells (AG-10200-200) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of PGE2. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of PGE2 in ACTOne PTGER4 cell line. EC50 = 26.2 pM in the presence of PDE inhibitor Ro20-1724, and EC50 = 121 pM in the absence of Ro20-1724 (Data not shown). B. Parental cells do not respond to PGE2 when the concentrations are less than 1 nM (in presence of PDE inhibitor). In the absence of PDE inhibitor, parental cells do not respond to PGE2 when the concentrations are less than 10 nM (Data not shown)