Neuropeptide Y Receptor Y2 cell line

No Description Available.

Figure 1. Response of ACTOne NPY2R cell line & parental cell line to NPY. ACTOne NPY2R cells and parental cells (AG-10200-200) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 35 min after the addition of NPY. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of NPY in ACTOne NPY2R cell line. EC50 = 1.2 nM in the presence of PDE inhibitor Ro20-1724 and b-adrenoceptor agonist isoproterenol. B. Parental cells do not respond to NPY.