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Immortalized Sweat Gland Myoepithelial Cell Line (iEM)

SKU : BHC10900933

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0816
Description	This novel, immortalized ecrine sweat gland myoepithelial cell line (iEM) serves as a model for research on sweat glands and sweating. These cells were developed from primary cells transduced with lentiviral vectors containing hTERT and SV40T. Cells display spheroid formation with increased population doubling compard to non-transduced cells. iEM's express myoepithelial cell-specific proteins (a-SMA, B1 or K14) and can differentiate into luminal cells. Immortalized Sweat Gland Myoepithelial Cells provide a useful?n vitro?odel for recapitulating eccrine sweat gland functions and for screening substances that can modulate sweating by acting on sweat glands. These cells are applicable for studies in hyperhidrosis, adiaphoresis, and sweat gland diseases. Please note: cells are exected to grow to 65 population doublings (PDs), however, the exact PD limit can vary by lot.
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	Osaka University
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Donor History	Female, 53, Eyelid
Expression Region	Alpha-SMA, puromycin resistance
Growth Properties	Suspension, round spheroid
Growth Conditions	Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids.?ammocult Human Medium Kit (Stemcell Technologies, Cat. 05620) + 10 ng/ml EGF (Z100139) + 10 ng/ml bFGF (Z101456) + 4 ?/ml heparin + 0.5 ?/ml hydrocortisone 21-hemisuccinate + 2% (v/v) growth factor reduced matrigel (optional) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase.?row cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form.?ubculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells/well.
Quantity	1x106 cells / 1.0 ml
Tissue	Sweat Gland
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Population Doubling	60-75 hours
Species	Pig (Porcine)
Propagation	Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 100 µM beta-mercaptoethanol + 10 µg/ml insulin + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media supplemented with 10% FBS. Centrifuge cells at 300xg for 5 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10? cells/ml. 2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900933	T0816	1x10^6 cells / 1.0 ml	-		Add to Quote

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