Immortalized Rat Neural Stem Cells (1464R)

Mfr.No.	T0746
Description	Immortalized Rat Neural Stem Cells (1464R) are spontaneously immortal population of neural stem cells derived from the brain stem. The cells retained morphology even after 50 passages. In the presence of retinoic acid, the cells may differentiate to TuJ1-positive neurons and to GFAP-positive astrocytes or O4-positive oligodendrocytes. May be used for brain disease studies, and a source for neuronal and glial cells.
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	Kyorin University
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Donor History	12 weeks old
Expression Level	p75-NGFr, Nestin, GFAP, Sulfatide O4
Growth Properties	Suspension
Growth Conditions	Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA, #P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA) + 10 ng/mL of FGF2 (Z101455) + 10 ng/mL of EGF (Z100139)? 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.?or information regarding cell differentiation, please refer to the "Differentiation Protocol" PDF under the Documents Tab.
Quantity	1x106 cells / 1.0 ml
Tissue	Brain
Morphology	Polygonal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Rat (R. norvegicus)
Population Doubling	20 - 30 hours
Species	Rat (R. norvegicus)
Propagation	Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, pituitary extract to a final concentration of 20 µg/ml, and forskolin to a final concentration of 2 µM.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Quality Control	Immunohistochemical staining of SV40 T antigen

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10? cells/ml. 2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHC10900922	T0746	1x10^6 cells / 1.0 ml	-		Add to Quote

Immortalized Rat Neural Stem Cells (1464R)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Immortalized Rat Neural Stem Cells (1464R)

Rate it now..!!