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Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO)

SKU : BHC10902193

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0203
Description	These cells are made in the background of the C57Bl/6 CD45.1 congenic mouse strain (Mercier et al.) that was crossed with the estrogen-receptor knockout mouse strain (https://www.jax.org/strain/026176). The CD45.1 marker allows for easy in vivo tracking of the cells. The ER-KO was performed to control for any undesired effects of exogenous estradiol on the progenitor cell function. This panel of cell lines is suitable for immunological reasearch.?dditional cell lines within this panel include: Cat. T0200 - Immortalized Myeloid Granulocyte Cell Line (ECoM-G) Cat.?0201 - Immortalized Myeloid Monocyte Cell Line (ECoM-M) Cat.?0202 - Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO) Cat.?0203 - Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO) Cat.?0204 - Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP) Cat.?0205 - Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP) Cat.?0207 - Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57) Cat.?0209 - Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	University of California, San Diego
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Region	STEM (CD45.1) and ER knockout
Growth Properties	Half suspension/ half adherent
Growth Conditions	PriCoat?T25 Flasks (G299) are recommended for optimal cell culture;?assage to new plastic with fresh media at least every 4 days.?he base medium for this cell line PriGrow II (TM002) supplemented with 10% FBS + 10 ng/mL GM-CSF + 0.5 ?M ?estradiol (0.5 ?M) + 1% Penicillin/Streptomycin (G255),37.0?, 5% CO?.?-estradiol is required for cell maintenance but must be completely removed for cell differentiation.Cells are sensitive to post-thaw conditions but recover after 24 hours in culture.For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.
Tissue	Bone Marrow
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Mouse (M. musculus)
Cryopreservation	10% DMSO + 50% FBS + 40% Complete Growth Media.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 500xg for 5 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Transfer susepension culture into a sterile centrifue tube, then add 2-3ml of pre-warmed 0.35% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-3 minutes). Cells that are difficult to detach can be put in 37?, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into the sterile centrifuge tube, and centrifuge at 500-600xg for 5 minutes.?br>5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10902193	T0203	1x10^6 cells / 1.0 ml	-		Add to Quote

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